Immunohistochemistry (IHC) is the most widely used diagnostic technique in tissue pathology, and antibody detection via imaging technology is a staple in any lab.  

However, IHC is associated with several limitations including low-plex count and lack of quantification. The limited multiplexing results in missed opportunities to ask key questions about tumor biology that could be important for diagnostic and prognostic outcome. 

This webinar will dive into a brief history of antibody detection, the current state of detection, and how to validate your fluorescently tagged antibodies for use in high resolution and high sensitivity applications such as with the CellScape™ ChipCytometry™ instrument.

Webinar Learning Objectives:

• Proper methods for antibody validation for various applications with a focus on directly conjugated fluorescent probes.
• How the ChipCytometry platform employs high-resolution HDR imaging for single-cell quantification of both high- and low-expressing targets
• The ChipCytometry workflow for high-plex quantification of protein targets using standard open-source antibody conjugations.