Navigating the multiplex image analysis workflow: from images to data-driven insights


An increasing need for the discovery of biomarkers and identification of signatures and clinically relevant targets has emerged in recent years

Spatial biology enables the interrogation of multiple biomarkers while keeping the information of spatial tissue architecture.

Transforming the multidimensional output of multiplex images into meaningful quantitative data is the next significant hurdle to fully comprehending and interpreting the intricate imaging data. 

In this webinar, Dr. Navikas delves into the realm of hyperplex image analysis in spatial biology, offering valuable insights for researchers starting to generate and analyze hyperplex immunofluorescent images. In the second step, he will illustrate the essential steps in an image analysis workflow using Lunaphore's end-to-end solution as an example. 

The solution of Lunaphore includes COMET™, the staining and imaging platform, and HORIZON™, the analysis software. Here, he will present how HORIZON™ is an image analysis software tailored to COMET™ hyperplex immunofluorescence images. It seamlessly extends the COMET™ workflow, smoothly handling large data sets. 

With its straightforward and user-friendly approach, this analysis tool supports cell-based analysis and provides researchers with a toolset for early and high-level assessment of data in a hyperplex immunofluorescent image. 

He will walk through the image analysis workflow on HORIZON™ from a COMET™ image to biological insights to characterize cellular tissue architecture.

Webinar learning objectives:

  • Learn how to unravel the intricate molecular signatures thanks to analyzing spatial biomarkers of hyperplex images.
  • Discover how to transform multiplex data into multi-insight conclusions in a few clicks using a seamless image analysis workflow with HORIZON™ .
  • Explore how to choose and perform the best approach for single-cell phenotyping, from nuclei segmentation, cell clustering to phenotype visualization.

Dr. Vytautas Navikas

Application Development Scientist, Lunaphore

Vytautas has an extensive background in state-of-the-art imaging modalities and truly believes that seeing is believing.
During his studies, he worked on developing and applying various cutting-edge single-cell imaging techniques ranging from scanning-probe microscopy to single-molecule localization microscopy.
He graduated with a Ph.D. in Biotechnology and Bioengineering from Ecole Polytechnique Fédérale de Lausanne (EPFL) in 2021.
Vytautas joined Lunaphore in 2022 and since then he has been working on bringing the analysis tools for multiplex immunofluorescence images to accelerate the adoption of spatial biology.